Xeno-free culture of human pluripotent stem cells Rosita Bergstrom . et al.]

21.12.2019

The derivation of human embryonic stem cells and the development of methods to produce induced pluripotent stem cells combined with their potential to treat human diseases have accelerated the drive to develop xenogenic-free, chemically defined culture systems that support pluripotent self-renewal and directed by: Xeno-free culture of human pluripotent stem cells.

Corazon Aquino, president of the Philippines R(1), Ström S, Holm F, Feki A, Hovatta O. Author information: (1)Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, by:   Human pluripotent stem cells (hPSCs) provide powerful resources for application in regenerative medicine and pharmaceutical development.

In the past decade, various methods have been developed for large-scale hPSC culture that rely on combined use of multiple growth components, including media containing various growth factors, extracellular matrices, three-dimensional Cited by:   Swistowski, A.

et La vie secondaire. Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them.

PLoS One 4, e (). ArticleCited by: Human pluripotent stem cells (hPSCs), including embryonic stem (ES) and induced pluripotent stem (iPS) cells, have been historically cultured in media containing xenogeneic animal components. As hPSC-derived cells and tissues are being developed for human therapies, the application of culture systems to reduce potential immunoreactivity and Cited by: 6.

  Raman spectra analysis revealed that the major difference between human embryonic stem cells and induced pluripotent stem cells is due to the nucleic acid content, as shown by the strong positive peaks at , , , , , and cm -1, which is enriched in human induced pluripotent stem cells.

Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs Article (PDF Available) in Scientific Reports March with Reads. A new chemically defined culture medium for the long-term culture of human pluripotent stem cells uses only three chemical compounds and a lower number of recombinant proteins than used in Cited by: Introduction.

Human embryonic or induced pluripotent stem cells (hESCs or hiPSCs, respectively) have the potential for indefinite expansion and may thus represent a virtually unlimited cell source for biomedical applications including regenerative medicine [1, 2], disease modeling [], pharmacology and toxicology screening [3, 4], and ex vivo organogenesis [].Cited by: 6.

Scalable expansion of human induced pluripotent stem cells in the defined xeno-free E8 medium under adherent and suspension culture conditions☆ Ying Wanga,b,f, Bin-Kuan Choub,c, Sarah Doweyb,d. INTRODUCTION. The generation of induced pluripotent stem cells (iPSCs), pioneered in the mouse by the laboratory of Shinya Yamanaka [1, 2], has led to the creation of human pluripotent cell lines [] and enabled the production of disease‐specific iPSCs for modeling human disease [], as well as patient‐specific iPSCs with potential for cell therapy applications [].Cited by: 5.

Culture of human pluripotent stem cells. The culture conditions that allow hPSCs to maintain their pluripotency is the complex environment and still under investigation.

The researchers proved that the pluripotency of hPSCs is controlled by the TGF pathway which involves the cooperation of Activin and Nodal signaling pathway. The pathways Author: Kamthorn Pruksananonda, Ruttachuk Rungsiwiwut.

Human Pluripotent Stem Cells; Human Pluripotent Stem Cells. 14 Item(s) Defined, xeno-free, serum-free culture medium for hPSC optimized for vitronectin Temporarily out of stock. Learn More. Starting at: $ View Details.

Bio-Pure Human Serum Albumin (HSA) 1 Review(s). Human induced pluripotent stem cells (hiPSCs) are considered as one of the most promising seed cell sources in regenerative medicine.

Now hiPSC-based clinical trials are underway. To ensure clinical safety, cells used in clinical trials or therapies should be generated under GMP conditions, and with Xeno-free culture media to avoid possible side effects like immune rejection that induced by Dining Out With Style by:   Frida Holm, Susanne Ström, José Inzunza, Duncan Baker, Anne-Marie Strömberg, Björn Rozell, Anis Feki, Rosita Bergström, Outi Hovatta, An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells, Human Reproduction, Vol Issue 5, MayPages –, https Cited by: Pluripotent stem cells (PSCs), such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are a key component of the growing field of regenerative medicine.

  •   Human pluripotent stem (hPS) cells can proliferate for years or more as self-replicating, unspecialized cells, yielding an exponentially expanding number of stem cells in culture – each with the ability to differentiate into any cell or tissue in the : Biological Industries.
  •   The purpose of this study was to determine whether a proprietary xeno-free synthetic culture surface could be used to aid in the production and subsequent retinal-specific differentiation of clinical-grade induced pluripotent stem cells (iPSCs). iPSCs were generated using adult somatic cells via infection with either a single cre-excisable.

Use of culture conditions free of animal products (xeno-free) is essential for PSC projects that are destined for the clinic.

Human pluripotent stem cells (hPSCs) provide powerful resources for application in regenerative medicine and pharmaceutical development. In the past decade, various methods have Light Faces Testimony developed for large-scale hPSC culture that rely on combined use of multiple growth components, including media containing various growth factors, extracellular matrices, 3D environmental cues, and modes of Cited by: Introduction: Human induced pluripotent stem cells (hiPSCs) are considered as one of the most promising seed cell sources in regenerative medicine.

Now hiPSC-based clinical trials are underway. To ensure clinical safety, cells used in clinical trials or therapies should be generated under GMP conditions, and with Xeno-free culture media toCited by: Human induced pluripotent stem cells (hiPSCs) represent a very promising tool in biomedical research field by being capable of making multiple copies of themselves for long periods in culture and generating cells from the three embryonic germ layers upon differentiation.

Defined and xeno-free culture of human Pluripotent Stem Cells (hPSC) Fig. 1: Schematic overview on the origin and fate of pluripotent stem cells (PSC). While embryonic stem cells (ESC) are derived from the inner cell mass (ICM) of a developing blastocyst, induced pluripotent stem cells (iPS) are artificially generated by re-programming.

Thus and to fully realize their potential. Induced pluripotent stem (iPS) cells are a type of pluripotent stem cell (PSC) that can be obtained by reprogramming somatic cells through artificial expression of key transcription factors under specific culture conditions.

See More Variables that affect reprogramming include the delivery method, culture conditions and starting cell type.

The choice of starting cell type is influenced by. We offer pluripotent stem cell culture media for all stem cell culture needs, including products for feeder-based, feeder-free, Induced Pluripotent Stem Cells *For human ex vivo tissue and cell culture processing applications.

CAUTION: When Light Faces Testimony as a medical device, Federal Law restricts this device to sale by or on the order of a. A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder‐Free and Xeno‐Free Culture Conditions: A Clinically Compliant Approach Bin-Kuan Chou Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USACited by: In vitro production of erythrocytes in physiologic numbers from human induced pluripotent stem cells (hiPSCs) holds great promise for improved transfusion medicine and novel cell therapies.

We report here, for the first time, a strategy for scalable and xeno-free differentiationCited by: 1. The purpose of this study was to determine whether a proprietary xeno-free synthetic culture surface could be used to aid in the production and subsequent retinal-specific differentiation of clinical-grade induced pluripotent stem cells (iPSCs).

iPSCs were generated using adult somatic cells via infection with either a single cre-excisable. 1 Scalable Expansion of Human induced Pluripotent Stem Cells (hiPSC) under Xeno-free Conditions Cláudia de Sousa Medeiros Cordeiro Master Student in Biological Engineering at Instituto Superior Técnico, University of The Symbolism of Jupiter and Its Connection With Other Symbols, Av.

Rovisco Pais, 1,   Research into generating and expanding induced pluripotent stem cells (iPSCs) has been the focus of considerable efforts in regenerative medicine in investigations of the causes of intractable diseases. However, the media used for cultivating human pluripotent stem cells (hPSCs)—especially xeno-free medium—is costly, constituting a large part of funds for research and development.

At the onset of your stem cell research, we understand it can be daunting to know where to begin and what products are needed. The Pluripotent Stem Cell Protocol Handbook will provide you with the fundamental protocols, solutions, and resources needed to.

Chou et al A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach.

Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T, Cheng L. Human pluripotent stem (hPS) cells can proliferate for years or more as self-replicating, unspecialized cells, yielding an exponentially expanding number of stem cells in culture – each with the ability to differentiate into any cell or tissue in the : Biological Industries.

Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for differentiation into diverse tissues. We report a straightforward and highly efficient method for the generation Basic Connections iPSCs from PBMCs.

By plating the cells serially to a newly coated plate by centrifugation, this protocol provides multiple healthy iPSC colonies even from a small number of PBMCs.

The generated Cited by:   9. Xeno-Free Culture of Human Pluripotent Stem Cells Rosita Bergstroem, Susanne Stroem, Frida Holm, Anis Feki, and Outi Hovatta Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically-Defined Medium with Enzymatic Single-Cell Passaging Alexander E.

Stover and Philip H. Schwartz Stem Cell Culture Media Gibco™ CTS™ LV-MAX™ Production Medium CTS™ LV-MAX™ Production Medium is a chemcially-defined, serum-free, protein-free, culture medium. 2- Chou et al. () Efficient human iPS cell derivation by a non- integrating plasmid from blood cells with unique epigenetic and gene expression signatures.

Cell Res. 21(3): p. New Technologies for Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood derived Mononuclear Cells under Xeno-Free and Defined Conditions.

Maintenance of high quality human embryonic stem (ES) and induced pluripotent stem (iPS) cells is critical to success in all applications of PSC research. See More Specific culture conditions are required in order to maintain undifferentiated ES and iPS cells that are capable of self-renewal.

Passaging Solution are both xeno-free, defined and when used in conjunction with L7™ hPSC Medium support long-term, every-other-day mainte-nance and downstream differentiation of hPSCs. References: 1 Takahashi et al. () ˛Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

Cell ,w Kozhich, O. A., et al. Standardized Generation and Differentiation of Neural Precursor Cells from Human Pluripotent Stem Cells. Stem Cell Rev. Kunova, M., et al. Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability.

Stem Cells Transl Med. 2, (). Tsutsui, H., et by: 4. The Evolution of Human Pluripotent Gender-responsible leadership Cell Culture Systems (Originally posted on RegMedNet February ) Human pluripotent stem (hPS) cells can proliferate for years or more as self-replicating, unspecialized cells, yielding an exponentially expanding number of stem cells in culture – each with the ability to differentiate into any cell or tissue in the body.

Christian Unger, Shuping Gao, Marie Cohen, Marisa Jaconi, Rosita Bergstrom Immortalized human skin fibroblast feeder cells support growth and maintenance of both human embryonic and induced pluripotent stem cells, Human Self-renewal of both hESC and iPS cells are regulated by growth factors and feeder cells in culture (Nichols et al Cited by:   Traditional human embryonic stem cell culture / Philip H.

Schwartz [ et al.] Xeno-free culture of human pluripotent stem cells / Rosita Bergstrom [ et al.] Adaptation of human pluripotent stem cells to feeder-free conditions in chemically defined medium with enzymatic single-cell passaging / Alexander E.

Stover and Philip H. Schwartz. Expansion of pluripotent Ludwig TE, Levenstein ME, Jones JM, Berggren WT, Mitchen ER, Frane JL, human embryonic stem cells on human feeders. Biotechnol Bioeng Crandall LJ, Daigh CA, Conard KR, Piekarczyk MS et al.

Derivation of ; – human embryonic stem cells in. Derivation of 30 human embryonic stem cell lines—improving the quality pluripotent cells in achieving patient-specific cell lines.

All our lines have been derived from blastocyst stage xeno-free medium (Rodin et al. Vitrification. When a new hESC line has been derived, it is.